Cui Daxiang
Published:2014-03-07 Hits:3005

Cui Daxaing, distinguished professor of shanghai jiaotong university, vice dean of Translational Medicine Research Institute of Shanghai Jiaotong University, the head of Nano-Biotechnology Research Institute, Assistant Dean of Institute of micro-and nano-technology, PhD supervisor, Deputy Director of the key laboratory of thin-film micro-technology, winner of the National Fund for distinguished young scholars, Chief Scientist of Nano major scientific research projects. Achieve the Bachelor of medicine degree at the second military medical University in 1990, the Master's degree in medicine at the fourth military medical University in 1995, the PhD in Biochemistry and molecular biology in 1998. Work in the institute of gene diagnosis technical application Research of the fourth military medical university and been promoted to associate professor in 2000. He achieved postdoctoral fellow Germany Max Planck Institute between 2001 and 2004. He was involved in the formation of bio-nano-engineered of Max Planck Center and got the qualifications of Germany Project Manager and bio-safety management certificates. He was responsible for the bio-safety of bio-nano-engineered center, and the head of bio-nano lab group. After returning to china in September 2004, he was hired as a professor of Institute of micro-and Nano-Science and technology of Shanghai Jiao Tong University and set up nano-bio-engineering research center and has been the Director of the Research Office.  Get the honour of Shanghai Pujiang talent in 2006, new century excellent talents of the Ministry of education in2008 and the Shanghai excellent academic leader in 2009. He was Japan's Waseda University visiting professor from September 2007 to March 2008. Research interests: nano-materials synthesis, biological effects and safety evaluation; the early diagnosis system of tumors based on nano-particle marking and nano-particle effects, multifunctional Nano-Probes and molecular imaging; tumor-related genes and protein structure and function ; CIK and stem cell therapy; efficient gene or drug delivery systems.
  He published more than 190 SCI papers in the international professional journals, such as Adv Mater., Nano Letters, Cancer Res, Molecular Therapy, Biomaterials, Plos One, small, Adv. Fun. Mater., Biomacromolecules, Anal. Chem., Chemical Communication, Biosensors&Bioelectronics, and Applied Physics Letters. His papers was cited by others more than 100 times in 4 of it, and one of his paper is cited more than 400 times. His papers are cited and commented by Nature Nanotechnology, Nature Materials and Chem. Review, and cited by others more than 3100 times, and H-index is 31. He was invited to report in international academic meeting more than 20 times and host the IEEE second session namo –biological medical international meeting as the Program Chair. He was awarded the second prize of army technology progress, the second prize of Shaanxi Province technology progress, the second prize of Shanxi Province technology progress and achieved one European and United States Patent and 22 China patents. He published 8 monographs in which 5were English monographs. He participated in the development of medical devices and achieve 5 certificate.
  Part Time in academic: standing member of China Nano Biotechnology branch, Vice Chairman of nano tumors branch of China anti-cancer Association, editor of Nano Biomedicine and Engineering magazine, Editorial Board of Nanoscale Research letters, Theranostics, Journal of Experiment Nanoscience, Journal of Oncology. He was member of biomedical photonics professional Committee and national standardization Technical Committee.
  In recent five years, in order to solve the key scientific issues of early warning and early diagnosis of gastric cancer, he carried out the screening of candidates for gastric cancer related markers, the principle of new technologies of nanometer detection, the development of multifunctional gastric carcinoma-targeted Nano-Probes, controllable preparation of nano-particles and study on application of nanometer detection devices. His main scholarly contributions are as follows:
1. screening and identification of markers for gastric cancer
He demonstrated that the Phospholipase c, Epsilon 1 (PLC E1) is low or no expression in lesions of atrophic gastritis, and high-level expression in gastric carcinoma. It’s an important molecular marker and can be used to distinguish early gastric cancer and atrophic gastritis. The papers published in PLos One 2012;e47563. He filterd out some respiratory gas markers that exist only in patients with gastric cancer though collecting more than 200 cases of respiratory gas of patients with gastric carcinoma and precancerous lesions. He conducted the hyper-sensitive method of breathing gases electrochemical sensor detection and applied for an international patent (PCT/CN2012/000083). The paper published in Theranostics 2013, Nanoscale Research Letters 2012;7:475.
2. Establishment of 4 principles of nano-probing technology
In order to improve the sensitivity and specificity blood markers of gastric cancer, he firstly reported the principle of hyper-sensitive technology of detecting nucleic acid and Antigen based on self-assembled quantum dots and carbon nanotubes. The oligonucleotide concentration that can be detected is 0.2pM, and Antigen concentration is 0.01nM, and the sensitivity and specificity is over ELISA methods. The multicomponent simultaneous detection can be realized because of different colors depended on the quantum dot size. The paper published in Analytical Chemistry (2008;80:7996-8001). Nature Nanotechnology listed it as Research Highlight and commented it on column of Top Down and Bottom Up in the 2008 period. It says “carbon nanotubes combined quantum dots can be highly sensitive to detect DNA and Antigen” “this system can be extended to biosensor applications”. He firstly reported the phenomena that quantum dots modified by mercaptoacetic acid can enhance the specificity of PCR and the principles of quantitative PCR method based on quantum dot fluorescence quenching, and applied the patents(ZL200610026091.1), which is more significant for testing of micro nucleic acid. He firstly reported the pollution-free and controlled synthesis of nano silver based bacteria as the template which is linked with Raman molecular whose detection signals can be increase 1011 times. The paper is published in Green Chemistry (2010;12:2038-2042). In order to detect out minor amount of maker in saliva, he conduct a method to detect out the microscale antibody in saliva by magnetic Nano particle mission clusters which combines fluorescent quenching fight gene probe and double features protein that can combine Fc fragments of antibody and has the features of MboI enzyme cutting. The method’s sensitivity can reached 0.1ng/mL which is significant to the detection of early and few maker of AIDS HIV, hepatitis virus, and tumor. The paper is published in Analyst (2011; 136:679-683). He established the principles of quantitative PCR technology based on the fluorescence quenching of quantum dots and achieve the invention patents (ZL200610026091.1) which transferred to the Shanghai bio-pharmaceutical company and developed a series of kits.
3. the design and preparation of 3 types of Microfluidic magnetic sensors
In order to capture of gastric cancer cells in the circulating blood, he designed magnetic nanoparticle probes coated by Chitosan and modified by RGD , which is fixed on the surface of Microfluidic channels. The probes achieve the rapid acquisition and quantitative detection of gastric cancer cells in the blood using giant magnetoimpedance (GMI) effect. For cancer-related viruses such as HPV viruses, he designed the GMI sensor micro-fluidic chip system which achieved rapid genotyping of 16/18 HPV virus. For HBV virus, he designed a giant magneto-resistive (GMR) chip sensor which can detect 8 indicators at the same time by using magnetic nanoparticles clusters and combined with linear probes and PCR techniques. The probes achieved rapid genotyping of HBV. The papers are published in Lab-on, respectively a Chip (2012:12 (4): 741-745), Biosensors&Bioelectronics (2011;26:3246-3253), Applied Physics Letters (2010;97 (043702)).
 4. Develop 4 gastric carcinoma-targeted image multifunctional Nano-Probes
  In order to achieve the positioning quantitative Visualization of early gastric cancer, he prepared fluorescent magnetic Nano particle probe combined with BRCAA1 (AF208045) antibody which has autonomous intellectual property. The probe can actively target to 5mm diameter gastric cancer of nude rat model, and achieved fluorescent imaging and MRI of gastric cancer. The paper was published in Journal of Nanobiotechnology (2011;9:93), and he was invented to reported in fifth session European clinical Nano medical Conference. He firstly reported the new method of controlled synthesis of up-conversion fluorescent magnetic Nano particle by using double phase system including oil acid and ion liquid. The probes can achieve CT ,MRI and near infrared fluorescent imaging to the gastric cancer model. The up-conversion Nano particle(NaYF4:Yb,Er) modified by cribellate Silicon can achieve targeted therapy and near infrared fluorescent imaging of cancer, carrying tumor chemotherapy drug, under outside magnetic field. The paper is published in Chemical Communication (2011;47:9510), Adv. Fun. Mater. (2011;21:4470), Chemistry-a European Journal (2012;18:5954) and Nano Letters (2012; (12): 61). In order to achieve the integration of targeted imaging and treatment of gastric cancer, he developed near-infrared quantum dots doped with RNAse and combined with RGD or Her2 antibody, which can target actively to gastric carcinoma in situ and release RNAse , destroying mRNA, to inhibit the growth of tumor cell. The carbon probe linked with photosensitizer Ce6, laser-induced, can show enhanced fluorescence imaging and Photodynamic therapy. The papers are published in Small (2010;ll6:2367), and Biomaterials (2012;33:7093) and Advanced Materials (2012;24:5104). The gold Nanorods probe modified with RGD-dendrimer can target tumor blood vessels, irradiated by near infrared light ,kill cancer cells and extend the survival time of tumor-bearing mice significantly. Papers are published in Molecular Pharmaceutics(2010, 7(1):94)。The gold Nanorods probe modified by Silicon and connected with folic acid can significantly enhance the sensitivity of radiotherapy while achieving targeted imaging. The papers are published in Biomaterials (2011;32:9796). The gold Nano-prisms modified by PEG achieved photoacoustic imaging of the gastrointestinal cance. The papers are published in the Small (2013;9:68-74). The hydroxyapatite rods modified by Eu (3+)/Gd (3+) achieved fluorescence, CT and MRI imaging, drug delivery and treatment. The papers are published in Biomaterials (2011;32:9031). He firstly proved the marked embryonic stem cells and IPS cells marked by fluorescent magnetic nanoparticles can identify actively gastric cancer cells in the body. The papers are published in Theranostics (2012;2:618-628). He applied patents and invited to made a report in the 449 session Beijing xiang-hills meeting.
5、Develop 3 nano-drug delivery system with high performance
The magnetic particles modified by dendrimer and cell penetrating peptide can take antisense or siRNA into tumor cells and achieve Cellular Imaging, inhibit tumor growth. The papers are published in Cancer Research (2007;67:8156) with Biomacromolecules (2012; 13:2,723). He developed the paclitaxel-loaded polymeric block copolymer Nano-micelles with good stability and good targeting as drug delivery system. The papers are published in Biomaterials (2010;31:2302).
6、In the respect of nano diagnostic reagent and nano devices translation , he developed magnetic strips and strips of quantum dots ,using the technology of magnetic particles and quantum dot markers, and developed supporting test equipment. The sensitivity of the strips can reach to 5pg/ml and 20pg/ml respectively. The papers are published in J. Biomed.Nanotech. (2011;7:776;2012;8:372). The biological immune chromatographic chip detector was developed by medical device registration certificate (food machinery 2011 Su 2400762).
7、Established Nano Biomedicine and Engineering International magazine
Hosted projects:
(1) national nanotechnology research projects: To solve key scientific problems in early warning and early diagnosis of stomach cancer using of nanotechnology (No.2010CB933900), Chief Scientist and funds $ 25 million, January 2010 to December 2014.
(2) national science and technology major projects: the study of rapid field detection system based on nano-effects of nano-particles marker. (2009ZX10004-311), the project team leader and funds $ 8.46 million, January 2009 to December 2014 , end of projects .
(3) State "Eleven-Five" and 863 nano-biotechnology research: immune chromatography chip and Biosensor based on nanoparticles (No.2007AA022004), the responsible person and funds $ 2.1 million,  December 2007 to December 2010, end of projects.
(4) Shanghai Nano special emphasis funding: AIDS rapid detection kits and supporting Nano-device development (No.0752nm024), the responsible person and funds $ 900,000, September 2007 to September 2009, end of projects.
(5)National Natural Science Foundation projects:  full-length cDNA cloning of differentially expressed genes in 3 persons with gastric cancer and chromosome localization study (No.3990177), the man in charge and NT $ 180,000, from 1999 to 2001, end of projects.
(6) National Science Fund project: a functional study of the GCYS-20 gene (No.30070838), the man in charge and NT $ 220,000 yuan, from 2000 to 2002, end of projects

Talent planning project.
(1) the admission of talents scheme in new century of the Ministry of education in 2008(NCET-08-0350), and safety evaluation of biological effects of nanomaterials (NCET-08-0350), the head of Fund of 500,000 yuan, from January 2008 to December 2010, end of projects.
(2)Shanghai excellent academic leader plan in 2009 (10XD1406100), positioned and quantitative visualization research in molecular markers for gastric cancer in vivo (10XD1406100), the head of Fund of 200,000 yuan from March 2010 to March 2012. end of projects.
(3) Shanghai Pujiang program in 2006 (06PJ14049), the head of Fund of 300,000 yuan, from September 2006 to September 2008, end of projects.
(4) National Fund for distinguished young scholars in 2012: medical imaging and biomedical engineering (No.81225010) and NT $ 2 million, between January 2013 to December 2014.

Representative Papers
1. Huang P, Lin J, Wang XS, Wang K, Zhang CL, Wang Q, He M, LiZM, Chen F, Shen GX, Cui D, Chen X. Light-Triggered Theranostic Based on Photosensitizer-Conjugated Carbon Dots for Simultaneous Enhanced-Fluorescence Imaging and Photodynamic Therapy. Advanced Materials 2012; 2012, 24, 5104–5110.
2. Zhi X, Liu Q, Zhang X, Zhang Y, Feng J, Cui D. Quick genotyping detection of HBV by giant magnetoresistive biochip combined with PCR and line probe assay. Lab on A Chip 2012; 12(4):741-745.
3. Ruan J, Song H, Qian QR, Li C, Wang K, Bao C.C., Cui D. HER2 monoclonal antibody conjugated RNase-A-associated CdTe quantum dots for targeted imaging and therapy of gastric cancer. Biomaterials 2012; 33: 7093-7102.
4. Huang P, Omar P, Wang, X, Wang, Z, Li, Z, Zhang CL, Chen F, Jing L, Cui D., Chen X. Chiral guanosine 5'-monophosphate-capped gold nanoflowers: Controllable synthesis, characterization, surface-enhanced Raman scattering activity, cellular imaging and photothermal therapy. Nano Res. 2012; 5: 630-639.
5. Zhang F, Braun G. B., Pallaoro A, Zhang Y, Shi Y, Cui D, Moskovits M, Zhao D, and Stucky GD.. Mesoporous Multifunctional Upconversion Luminescent and Magnetic “Nanorattle” Materials for Targeted Chemotherapy. Nano Letters 2012;12(1): 61-67.
6. Qi L, Wu L, Zheng S, Wang Y, Fu H, Cui D. Cell-Penetrating Magnetic Nanoparticles for Highly Efficient Delivery and Intracellular Imaging of siRNA. Biomacromolecules 2012; 13(9): 2723-2730. 
7. Chen F, Huang P, Zhu Y, Wu J, Cui D. Multifunctional Eu3+/Gd3+ dual-doped calcium phosphate vesicle-like nanospheres for sustained drug release and imaging. Biomaterials 2012; 33:6447-6455.
8. Ruan J, Ji J, Song H, Qian Q, Wang K, Wang C, Cui D. Fluorescent magnetic nanoparticle-labeled mesenchymal stem cells for targeted imaging and hyperthermia therapy of in vivo gastric cancer. Nanoscale Res. Lett. 2012; 7: 309.
9. Ruan J, Song H, Wang K, Qian Q, Cui D. DiR-labeled Embryonic Stem Cells for Targeted Imaging of in vivo Gastric Cancer Cells. Theranostics 2012; 2:618-628.
10. Chen J, Wang W, Zhang T, Ji J, Qian Q, Lu L, Fu H, Cui D. Differential expression of phospholipase C epsilon 1 is associated with chronic atrophic gastritis and gastric cancer. Plos One 2012; 7(10): e47563.
11. He M, Huang P, Zhang C. L., Hu H. Y., Bao C. C., Gao G., Chen F, Wang C, Ma J. B., He R, Cui D X. Dual phase-controlled synthesis of uniform lanthanide-doped NaGdF4 upconversion nanocrystals via an OA/ionic liquid two-phase system for in vivo dual-modality imaging. Advanced Functional Materials 2011; 21: 4470 - 4477.
12. Zhang X, Jiang L, Zhang CL, Li D, Wang C, Gao F, Cui DX. A Silicon Dioxide Modified Magnetic Nanoparticles-Labeled Lateral Flow Strips for HBs Antigen. J. Biomed. Nanotechnol. 2011; 7: 776-781.
13. Ruan J, Shen J, Wang Z, Ji JJ, Song H, Wang K, Liu B, Li JH, Cui DX. Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology. International Journal of Nanomedicine 2011, 6, 425-435.
14. Huang P, Li ZM, Lin J, Yang DP, Gao G, Xu C, Bao L, Zhang CL, Wang K, Song H, Hu HY, Cui DX.Photosensitizer-conjugated magnetic nanoparticles for in vivo simultaneous magnetofluorescent imaging and targeting therapy. Biomaterials 2011, 32, 3447-3458.
15. Chen L, Bao CC, Yang H, Li D, Lei C, Wang T, Hu HY, He M, Zhou Y, Cui DX. A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells. Biosensors and Bioelectronics 2011; 26: 3246-3253.
16. Yang DP, Chen SH, Huang P, Wang XS, Jiang WQ, Pandoli O, Cui DX. Bacteria-template synthesized silver microspheres with hollow and porous structures as excellent SERS substrate. Green Chemistry 2011;12:2038-2042.
17. Kong YF, Chen J, Gao F, Li WT, Xu X, Pandoli O, Yang H, Ji JJ, Cui DX. A Multifunctional Ribonuclease-A-Conjugated CdTe Quantum Dot Cluster Nanosystem for Synchronous Cancer Imaging and Therapy. Small 2010, 6, 2367-2373.
18. Yang H, Chen, Lei C, Zhang J, Li D, Zhou ZM, Bao CC, Hu HY, Chen XA, Cui F, Zhang SX, Zhou Y, Cui DX. Giant magnetoimpedance-based microchannel system for quick and parallel genotyping of human papilloma virus type 16/18. Applied Physics Letters 2010, 97.
19.Song H, He R, Wang K, Ruan J, Bao CC, Li N, Ji JJ, Cui DX. Anti-HIF-1 alpha antibody-conjugated pluronic triblock copolymers encapsulated with Paclitaxel for tumor targeting therapy. Biomaterials 2010, 31, 2302-2312.
20. Li ZM, Huang P, Zhang XJ, Lin J, Yang S, Liu B, Gao F, Xi P, Ren QS, Cui DX. RGD-Conjugated Dendrimer-Modified Gold Nanorods for in Vivo Tumor Targeting and Photothermal Therapy. Molecular Pharmaceutics 2010, 7, 94-104.
21. Cui DX, Pan BF, Zhang H, Gao F, Wu R, Wang JP, He R, Asahi T. Self-Assembly of Quantum Dots and Carbon Nanotubes for Ultrasensitive DNA and Antigen Detection. Analytical Chemistry 2008, 80, 7996-8001.
22. Pan BF, Cui DX, Sheng Y, Ozkan CG, Gao F, He R, Li Q, Xu P, Huang T. Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system. Cancer Research 2007, 67, 8156-8163.
23. Ao LM, Gao F, Pan BF, He R, Cui DX. Fluoroimmunoassay for antigen based on fluorescence quenching signal of gold nanoparticles. Analytical Chemistry 2006, 78, 1104-1106.
24. Cui DX, Jin GQ, Gao TW, Sun TB, Tian FR, Estrada GG, Gao HJ, Sarai A. Characterization of BRCAA1 and its novel antigen epitope identification. Cancer Epidemiology Biomarkers and Prevention 2004, 13, 1136-1145.
25. Huang P, Bao L, Zhang C .L, Lin J, Luo T, Gao B, Fu F, Cui D. X. Folic Acid-Conjugated Silica-Modified Gold Nanorods: Enhancement of Cell Radiation Sensitivity, Photo-Thermal Therapy, and Bio-Imaging. Nanoscale 2011; 3(9):3623-3626.

Research group of Shanghai ICP 11031250 -1 2013 Shanghai Jiao Tong University pollution control

Support by: Wei Cheng